Objective 5 and Deliverables of WP5
Α Cdc6 construct [CAG-loxP-eGFP-STOP-loxp-Cdc6-IRES-βGal (CAG-Cdc6)] was injected in zygote pronuclei. Following implantation in pseudopregnant females resulted in generation of the first known CAG-Cdc6 founders. Currently, these founders are undergoing crossings with C57BL/6J mice to obtain the CAG-Cdc6 mouse model. These mice will be crossed with whole body or tissue specific Cre animals to obtain corresponding Cdc6 overexpressing animal models.